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A platform to study platelet aggregation and thrombus growth based on dynamic stress
F. J. Tovar-Lopeza1, G. Rosengarten2, K. Khoshmanesh3 , E. Westein4 , S. P. Jackson4, Arnan Mitchell 1, and W. S. Nesbitt4. 1: School of Electrical and Computer Engineering, RMIT University, Melbourne, VIC 3001, Australia; 2: School of Mechanical and Manufacturing Engineering, The University of New South Wales UNSW, Sydne

We present a microfluidic device that is able to trigger initial recruitment and subsequent aggregation of discoid platelets by mimicking the effects of pathological changes in blood vessel geometry.

Sub-classification of colorectal cancer using surface antigen antibody microarray and fluorescence multiplexing
Jerry Zhou, Larissa Belov, Pauline Huang, Joo-Shik Shin, Michael J. Solomon, Pierre Chapuis, Les Bokey, Charles Chan and Richard I. Christopherson. THE UNIVERSITY OF SYDNEY SCHOOL OF MOLECULAR BIOSCIENCES

Colorectal cancer (CRC) is the second most frequent cause of cancer deaths in Australia. Even after resection up to 50% of patients relapse. In an attempt to prevent recurrences chemotherapy is administered to high risk patients. However, as few as 10-20% patients genuinely benefit because the clinical course for individuals with CRC remains difficult to predict, largely due to prognostically heterogeneous groups within same-stage tumour categories.

Development of a Fully Integrated Microfluidic Device for Electromodulated Liquid Chromatography with C4D Detection
Jeremy Galineau, Blanaid White, Aoife Morrin, Malcolm R. Smyth. Sensors and Separations Group, National Centre for Sensor Research, School of Chemical Sciences

Applications for microfluidic technologies in life science are expanding rapidly, and have the potential to impact enormously across a range of fields, from cell manipulation to separation science. In separation science their small channel dimensions make them ideal for high throughput separations, reducing sample volumes and solvent consumption.

Sequence-independent Selective Amplification of mRNAs over rRNAs
John Arrand1, Sim Sihota1, Wenbin Wei1 and Guido Krupp2.

Standard mRNA amplifications for "All-Exon" microarrays and for bacterial RNAs are impossible with small samples and with degraded RNAs, because removal of rRNAs must precede universal, non-selective RNA amplification. This pre-treatment with magnetic beads is cumbersome, requires high amounts of starting material, is not universal for all species and degraded RNAs are not suitable.

A Practical Microfluidic Device for Synthesis of Purified Monodisperse Micro-Alginate Beads (MABs) as Microcarriers of Gold Nanoparticles.
Paul-Emile POLENI, Serge OSTROVIDOV, Yasuyuki SAKAI and Teruo FUJII. Institute of Industrial Science, The University of Tokyo

We developed a microfluidic device for encapsulating gold nanoparticles in Micro-Alginate Beads (MABs). The size and the gap of the monodisperse alginate droplets were successfully controlled by adjusting the relative "oil/sample" flow rate ratio. Droplets reacted with calcium ion at the interface between oil phase and aqueous phase so that MABs precipitated spontaneously and undergone complete gelation. Purified MABs were successfully observed by fluorescence microscopy.

ON CHIP PROTEIN DYNAMICS IN SINGLE BACTERIA CELLS WITH SPATIO-TEMPORAL RESOLUTION
Dominik Greif, Nataliya Pobigaylo, Anke Becker, Jan Regtmeier and Dario Anselmetti. Bielefeld University, Freiburg University, Germany

We demonstrate spatio-temporal protein dynamics in single living bacterial cells from time lapse fluorescence imaging (TLFI) in a microfluidic chip.

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