Latest Posters

Combination of a transient assay based ChIP technology and transcriptome analyses for the exploration of potential transcription factor binding sites
Shu-Jen Chou (presenter), Li-Teh Chen, Yi-Hang Li, Chiung-swey Joanne Chang and Shu-Hsing Wu,
Institute of Plant and Microbial Biology, Academia Sinica

1. Combination of protoplast transient expression system and Nickel resin based purification is an useful approach for chromatin enrichment that can be used to identify transcription factor’s potential target genes. 2.Comparison our ChIP-on-chip target genes with in vivo binding targets using two whole genome tiling array platforms showing high degree of overlap between two methods.

Phase Diagram Visualization via Continuously-Fed Crystallization: Experiments and Model
Masano Sugiyama and Victor Barocas, University of Minnesota

A continuously-fed crystallization chamber is manufactured to allow phase diagram visualization. This microfluidic system allows the experimenter to screen a large range of salt and protein concentrations in one experiment. This allows the experimenter to predict the protein phase diagram in a single experiment. A continuous-feed crystallization chamber has been successfully fabricated and characterized in terms of its flow profile, and has successfully predicted the phase diagram for lysozyme.

Microfluidic sensor device for online haemostasis monitoring
L. Müller, A. Sterck, R. Gronmaier, S. Sinn, D. Klar, S. Haeberle, R. Zengerle, H.P. Wendel, H. Northoff, F. Gehring,
University Hospital of Tuebingen, Institute of clinical and experimental transfusion medicine

We have developed a microfluidic chip that allows online haemostasis monitoring using a thickness shear mode sensor. The sensor allows the detection of adsorbed masses and changes in viscosity in real-time. The microfluidic mixer permits fast mixing of whole blood with activators, which trigger the coagulation cascade. Changes in frequency were measured during the coagulation of whole blood.

A microfluidic approach for the directed evolution of proteins by retroviral display
Lucia Granieri, Jean-Christophe Baret, Andrew D. Griffiths and Christoph A. Merten,
ISIS

The model system used here is based on retroviral particles displaying tPA, a protein used in current emergency therapies of myocardial infarction and stroke. Single tPA variants were encapsulated into aqueous droplets, at a frequency of ~10Kilohertz and the enzymatic activity was monitored using a fluorescence assay. Active variants could be clearly distinguished from inactive variants or variants incubated with the endogenous inhibitor PAI-1.

A high-throughput protein array-based approach
Rachel Van Dyk, Claudia Kirisits, Paul Potter, Fook Tim Chew, Reinhard Hiller,
Centre for Proteomic and Genomic Research

Here we present a novel discovery-oriented high-throughput approach to the screening of allergen reactivates in crude biological extracts is presented using seafood allergens as an example. The CPGR workflow permits the effective screening of hundreds of putative allergens in parallel using minute amounts of patient serum and constitutes a cost-efficient allergen-specific antibody screening method for a routine diagnostic setting.

Development of a High-Throughput Microarray for Evaluating CYP1A1 Induction
Megan Mason, XinXin Ding, Jonathan S. Dordick,
Rensselaer Polytechnic Institute

The aim of this study is to produce a vector capable of expressing green fluorescent protein (GFP) when exposed to a CYP1A1 inducer.